Imunoexpressão de CXCL12, CXCR4 e densidade microvascular em adenomas pleomórficos e carcinomas mucoepidermoides de glândulas salivares

Abstract

The aim of this study was to evaluate the immunohistochemical expression of the CXCL12 chemokine and its CXCR4 receptor in pleomorphic adenomas (PAs) and mucoepidermoid carcinomas (MECs) of the salivary glands, relating it to clinical and pathological parameters and to tumor angiogenic activity through microvascular density (MVD). Through the immunoperoxidase method, with antibodies anti- CXCL12, anti-CXCR4 and anti-CD31, under light microscopy, it has been established percentages of neoplastic cells immunostained in the intratumoral and peritumoral regions of PAs and MECs of salivary glands. Most of the PAs and all of the MECs were positive for CXCL12, with a statistically relevant difference in the expression of this protein related to the neoplasia type. A positive correlation was observed in the CXCL12 expression between the intratumoral and peritumoral regions of the sample. All of the APs and MECs were positive for CXCR4, showing a discrete statistically relevant difference in the expression of this protein in the peritumoral region related to the neoplasia type. Positive correlation of CXCR4 expression was observed between the intratumoral and peritumoral regions of the sample. It was found a statistically relevant difference of the MDV in relation to the neoplasia type, mainly about the MECs. Positive correlation of MDV was observed between the intratumoral and peritumoral regions of PAs and MECs. There was similar expression of the CXCL12 and the CXCR4, but the CXCL12/CXCR4 complex does not appear to be a meaningful factor in relation to the angiogenic tumoral activity. The most intratumoral MDV in MECs and peritumoral in APs probably suffer the influence of other factors or synergistic phenomena to the CXCL12/CXCR4 complex activation.