Avaliação do potencial antioxidante e osteoindutor do extrato do mastruz

Abstract

Considering the use of Chenopodium ambrosioides L. in folk medicine for the treatment of bone fractures, and the knowledge that antioxidants may be related to bone health, this study was developed to examine the antioxidant activity and the influence of ethanol extract of leaves of Chenopodium ambrosioides L. (EEC) on the in vitro differentiation of MC3T3-E1 pre-osteoblastic cells. Phytochemical screening was performed for quantification of flavonoids, polyphenols and tannins and thus it was found that the EEC has a high tannin content (43.27% catechin equivalents) and the remaining classes are well represented (6.57% for polyphenols and 6, flavonides to 24%). The antioxidant potential of the EEC was evaluated by total antioxidant activity (CAT), reducing power, kidnapping of superoxide radicals and copper chelation tests. For in vitro osteoblastic differentiation analysis, cell viability and proliferation tests were performed by reduction of tetrazolium dye (MTT), alkaline phosphatase (ALP) assay and observation of the degree of matrix mineralization by von Kossa staining. The EEC showed antioxidant potential, notably for tests that evaluated CAT (equivalent to 120mg of ascorbic acid) and copper chelation (79.2% of chelation compared to EDTA ). Furthermore, the EEC was able to positively affect MC3T3 cells proliferation, providing 251% in comparison with control in the first 24 hours, at a concentration of 150 µg/mL. However, in none of the tests for osteoblastic differentiation was observed positive influence of EEC for the treatments. Thus, the EEC presented as a powerful antioxidant, but did not reveal an osteoinductive property. So, the possible involvement of EEC in bone repair may be related to other pathways, including the antireabsortive and angiogenic potential, among others, which were out of scope of this work.