Avaliação da inibição de micro-organismos em resinas compostas Bulk Fill com uso de fitoterápico Zingiber officinale pelo sistema de tomografia por coerência óptica

Abstract

The oral cavity is one of the places where there is a greater variety of microorganisms that can, due to an imbalance, become pathogenic. The aim of this study was to evaluate the effects of Zingiber officinale phytotherapic on the growth and adherence of Staphylococcus aureus and Candida albicans microorganisms in vitro on smooth surfaces of high viscosity Bulk Fill composite resins and dental enamels, comparing their results with the application. chlorhexidine. For this purpose, an in vitro experimental laboratory study of the analytical type was carried out with two condensable Bulk Fill resins Tetric N-Ceram Bulk Fill - Ivoclar, Sonic Fill-Kerer and human enamel inoculating the groups. aforementioned microorganisms, using Zingiber officinale for growth inhibition as a positive control for chlorhexidine and as a negative control for non-intervention. In this study, two optical coherence tomography (OCT) systems were used: Callisto (Thorlabs Inc, New Jersey, USA) and LabScope (Lumedica, Durham, NC, USA), all samples were analyzed by OCT. The total sample consisted of 180 specimens, with n = 10 per group, and 10 images of each specimen and 30 images of each plating were obtained, both at three different times, sterile, grown and treated. Image processing was performed using an algorithm produced in MatLab language to determine the average signal strength and calculate optical density using the tiff images generated by OCT Labscope. The mean and standard deviation of each group were calculated using the Mann-Whitney and Kruskal Wallis tests. Through OCT evaluation, it was found that in the three surface types, both chlorhexidine and Zingiber officinale were effective in inhibiting S. Aureus and C. Albicans. The Tetric resin showed lower biofilm growth when compared to Sonic when treated with the analyzed rinses Zingiber officinale being an effective and viable method for microbial control and inhibition.