Filmes polimétricos de quitosana com um derivado n-acilidrazônico para tratar lesões cutâneas

Abstract

The N-acylhydrazone subunit is present in several bioactive molecules and in drug prototype candidates, but is still a poorly exploited compound. The association of an N-acylhydrazone derivative (JR19) with anti-inflammatory activity already studied with chitosan (CHI), a biopolymer with attractive pharmaceutical characteristics, may represent an innovative proposal for the treatment of topical inflammations in the form of films. The aim of this work was to develop, characterize physico-chemically and perform cytotoxicity study of CHI films with and without the incorporation of the molecule JR19. The films were developed from the sol-gel method and the techniques used for the proper characterization and other tests were of resistance to folding, traction test, swelling degree, wettability by contact angle measurements, optical microscopy (OM) scanning electron microscopy (SEM), roughness, analytical thin layer chromatography (ATLC), differential scanning calorimetry (DSC), thermogravimetry and its derivative (TG / DTG), Fourier transform infrared spectroscopy (FTIR) X-ray diffraction (XRD). In addition, agar diffusion analysis was performed for the in vitro cytotoxicity study. The films presented, from the results of MO, MEV and through the resistance test, the presence of crystals of the isolated molecule of JR19 when incorporated in the CHI film. In addition, they showed modifications of water absorption and mechanical strength of these films, when compared to CHI films without the incorporated JR19. The CHI + JR19 films showed compatibility in the DSC curves of the physical mixture [QTS: JR19 (1: 1)] and absence of chemical incompatibility of QTS films with JR19. The TG / DTG presented similar characteristics of loss of mass between the samples, prevailing to the characteristics of the CHI isolated in the films with JR19, the second stage (151.77-495.28 °C, Δm = 44.51 %) of the three displayed steps, the main one. The infrared spectra of the physical mixture and the film with the molecule exhibited bands characteristic of the functional groups of JR19 in the same absorption region, for example the detection of the nitrile stretch band (C≡N), which ranged from 2279 to 2264 cm-1 . The reflections observed in the diffractograms indicated decreased crystallinity of the films when compared to the physical mixture and omission of some reflections of JR19 in the films with the incorporated molecule. The evaluation of in vitro cytotoxicity by means of the agar diffusion method revealed quantitatively and qualitatively that the CHI and CHI + JR19 films presented a nontoxic character, a result that is satisfactory for future in vivo tests. Therefore, the techniques used allowed to characterize the formulation of CHI and JR19, showing very promising for the development of these films as a new proposal of pharmaceutical treatment in cutaneous lesions.