Propriedades antimicrobianas, mecânicas e biocompatibilidade de cimentos ionométricos modificados por própolis vermelha

Abstract

OBJECTIVE: To evaluate the antimicrobial activity, mechanical properties, fluoride release capacity and biocompatibility of glass ionomer cements (GICs) modified to contain the ethanolic extract of red propolis (EERP) at different concentrations. MATERIAL AND METHODS: Two orthodontic GICs containing EERP at 10%, 25% and 50%, were used. The following assays were carried out: cell viability tests against Streptococcus mutans and Candida albicans, diametral tensile strength (DTS), compressive strength (CS), shear bond strength, microhardness, fluoride release capacity, and morphological and immunohistochemical biocompatibility analysis in rat subcutaneous tissue. The statistical analysis of the antimicrobial tests, fluoride release, DTS, CS and microhardness was performed using Two -way ANOVA and Tukey's test (p<0.05). The shear bond strength data were analyzed by One-way ANOVA followed by Tukey's test (p<0.05). The morphological and immunohistochemical findings were analyzed by Kruskal-Wallis test followed by Dunn’s test (p<0.05). RESULTS: At the concentrations of 25% and 50%, EERP was shown to be a promising antimicrobial agent incorporated into GICs against C. albicans (P <0.001) and S. mutans (P <0.001). The fluoride ion release capacity of the GICs was not affected, and the EERP concentration of 25% was the one that least affected the mechanical properties of the cements (P> 0.05). In addition, the experimental cements showed biocompatibility in rat subcutaneous tissue. CONCLUSION: The GICs containing EERP at 25% showed a significant increase in their antimicrobial activity against S. mutans and C. albicans, unaltered mechanical properties, fluoride release capacity and tissue biocompatibility.