Potencial antifúngico e antibiofilme contra espécies de Candida e toxicidade da Anadenanthera colubrina vell. Brenan

Abstract

This study evaluated the antifungal and antibiofilm potential of the hydroalcoholic extract of the bark of Anadenanthera colubrina vell. Brenan, known as Angico and White Angico. The antifungal activity was initially evaluated by the microdilution technique in broth with minimum inhibitory concentration (MIC) and Minimum Fungicide Concentration (CFM). The potential antibiofilm was tested in mature biofilms formed by Candida species and analyzed by counts of CFU / mL and scanning electron micrograph (SEM). In vitro toxicity was analyzed in Raw 264.7 macrophages and systemic toxicity was evaluated in vivo in the Galleria mellonella model. A strong antifungal potential of the extract against strains of the genus Candida was observed, with the following MIC values: Candida albicans ATCC 5314 and ATCC 90028 (19.5 μg / mL), Candida tropicalis ATCC 750 (5000 μg / mL), Candida Glabrata ATCC 90030 (1250 μg / ml), Candida parapsilosis ATCC 22019 and Candida krusei ATCC 6258 (39 μg / ml). The treatment with the extract reduced the number of CFU / mL and was able to cause structural alteration in the biofilm and cellular destruction, observed in SEM, presenting a promossora antibiofilm activity against Candida. A. colubrina presented moderate toxicity in vitro in RAW 264.7 macrophages culture. In the in vivo assay the extract did not affect the viability of the larvae at doses below 100 mg / kg, presenting low toxicity. Considering its high antifungal potential and its low in vivo toxicity, A. colubrina extract is a strong candidate for the development of a new drug for the treatment of oral candidiasis.