Avaliação das atividades antimicrobiana, antioxidante e de citotoxidades de produtos extraídos da Agave sisalana Perrine

Abstract

Agave sisalana is a plant of Mexican origin that is characterized by providing the stiffest fiber in the world: the sisal. In Brazil, this plant is an important source of income for farmers in semi-arid regions mainly in the states of Bahia and Paraíba. The ability to create jobs is related to maintenance of the crops, harvesting, refining, processing fiber, industrialization and production of crafts. The aim of this study was to determine the antimicrobial, antioxidant and cytotoxicity of Agave sisalana Perrine in wastes obtained from the leaves of defibrillation. Antibacterial activity was determined against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and against fungiCandida albicans. The antioxidant activity was performed with the method of DPPH. The cytotoxicity was detected in HeLa cells using the cell proliferation reagent WST-1. The antibacterial activity was found in RL0

(E. coli, P. aeruginosa) and EEB-1 (S. aureus). Activity against C. albicans was found in RL-0, EEB-0 and EEB-1. Ethanol extracts have antioxidant activity, this analysis was not performed with liquid waste because they do not solubilized in ethanol. The cytotoxicity assay showed that the RL-0 is toxic at concentrations that have antimicrobial activity. The RL-1, EEB-0 and EEB-1, above extracts showed cell viability of the positive control (cells + DMSO), when the concentrations of 5 mg/ml, 1 mg/ml and 1 mg/mL, respectively. These results demonstrate that Agave sisalana can be a source of new therapeutic assets.