Novas propostas analíticas para qualificação de cumarina em amostras de xaropes fitoterápicos contendo guaco (Mikania glomerata)

Abstract

The species Mikania glomerata, popularly known as guaco, is widely used in our country in the treatment of various diseases, especially in respiratory problems, a fact that justifies the inclusion of this plant in SUS phytotherapy programs. Its main constituent is coumarin (1,2-benzopyrone), used as a chemical marker for the quality control of pharmaceutical formulations containing guaco. Thus, the present work aimed to develop two analytical strategies, following the principles of green chemistry for quantification of coumarin in commercial samples of phytotherapeutic syrups containing Mikania glomerata using UV-Vis spectroscopy and digital image analysis associated with multivariate calibration comparing Reference method (HPLC). The results obtained for the proposed methods stand out for their application range, since they were able to quantify coumarin in syrups composed of different matrices: one formulation containing sugar, another without sugar, intended for diabetics, and one containing other species Associated plants. The results are in agreement with those obtained for the reference method (HPLC), the great discrepancy observed in the assay of coumarin in the techniques used in relation to the contents declared on the labels of the products commercialized, which justifies the need to perform The quality control of these medicines in order to offer a safe and reliable product to consumers. Therefore, the methods proposed here for quantification of coumarin content in commercial samples of guano-containing herbal syrups (Mikania glomerata) are promising alternatives to the routine analysis performed by high-performance liquid chromatography, given their simplicity, low cost , Speed, robustness, and follow the Principles of Green Chemistry.