Potencialização da germinação e crescimento inicial de arroz vermelho inoculado com Gluconacetobacter diazotrophicus

Abstract

The utilization of Gluconacetobacter diazotrophicus in agriculture has shown promise in optimization morphophysiological aspects, biochemical and yield of plants. The culture of red rice (Oryza sativa L.) presents great socioeconomic and environmental importance in the semi- arid northeast. The production of this culture still suffers by the not full use of appropriate technologies, due to scarcity of studies and development of technologies. Given the above, the objective of this study was to evaluate the potential effect of G. diazotrophicus on germination and initial growth of red rice.The experiment was conducted in a completely randomized design, with six treatments (SNE-seeds not soaked in water, SE H2O-seeds soaked in water for 24 hours, SE H2O + GA3 seed soaked in gibberellic acid solution for 24 hours, SE H2O + GD-seeds soaked in water for 24 + G. diazotrophicus, SE H2O + GA3 + GD-seeds soaked in gibberellic acid solution for 24 hours + G. diazotrophicus and SNE + GD-seeds not soaked in water + G. diazotrophicus) with six replicates.The concentration of gibberellic acid (GA3) used was 50 mg -1 L . Evaluated were the aspects physiological, biochemical, molecular and initial growth of red rice seedlings. Data were submitted to analysis of variance, mean test and Pearson correlation. Treatments significant effect on the growth variables, physiological, biochemical and molecular.It was verified that treatment with non-imbibed seeds inoculated with G. diazotrophicus promote increase of 28.7; 41.7; 28.7; 49.5; 69.6; 48.5; 61.5; 38.5; 46; 97; 86 and 89% for the variables: germination, germination speed index, first count, root length, shoot length, total fresh mass, total dry mass of α-amylase activity, activ acid phosphatase, expression GAMYB transcription factor, α-amylase and S-adenosyl-L-methionine (SAM) synthase, respectively, in relation to the treatment of seeds not soaked in water and not inoculated.It was concluded that the inoculation with G. diazotrophicus red rice seeds enhances the speed of germination and seedling germination, root length, shoot length, and acid phosphatase activities of α-amylase, total fresh mass, total dry mass, GAMYB expression of the transcription factor, α- amylase expression and SAM. Therefore presents great potential agronomic and biotechnology for use as growth promoter in the red rice crop, increasing the germination and early growth independently of seed imbibition.