Potencial antimicrobiano e antiproliferativo da Anadenanthera colubrina (Vell.) Brenan

Abstract

This study aimed to evaluate the in vitro anti-microbial and anti-proliferative potentials of extract and active fractions of Anadenanthera colubrina (Vell.) Brenan (Angico). The anti-microbial evaluation was conducted through Minimum Inhibitory Concentration (MIC), Minimum Bactericidal/Fungicidal Concentration, and biofilm adherence inhibition, as well as a cellular morphology analysis via scanning electron microscope (SEM). To verify the anti-proliferative potential, six tumor cell lines were used. The extract presented severe anti-fungicidal activity against Candida albicans ATCC 18804 (MIC = 0,031 mg/mL), with similar activity in regard to its ethyl acetate fraction. Both the extract and the active fraction also showed the ability to inhibit Candida albicans adherence in biofilm after 48 hours of formation, showing median values equivalent and higher than those of the control group, but without significant statistical difference (p > 0,05). Through the SEM was also observed alterations in the cellular morphology of yeast. With regard to anti-proliferative activity, the extract presented cytostatic potential for all tested lines. In face of these results, it s suggested a strong anti-fungicidal inhibition potential for Anadenanthera colubrina (Vell.) Brenan, as well as a tendency for decreasing the cellular growth of human tumor lineages.